Figure 2: Summary of acetylation and phosphorylation sites identified by nLC-MS/MS in overexpressed rat ACSL1 in brown adipocytes in cell culture and in endogenous ACSL1 in mouse brown adipose tissue for all treatment groups. The sequence shown is rat ACSL1 (P18163). However, the amino acid residues in parenthesis indicate the sequence variations between rat and mouse that vary at the post-translational modification site. The numbers above the amino acids indicate the amino acid position of the modification; forward slashes are present to separate the numbers. The AMP binding site, Gate motif, Adenine binding motif, Fatty acid binding site, Linker region, and the consensus fragment of Motif V are underlined; Roman numerals designate the motifs, as described in reference [27].