Figure 3: ACSL activity change by ACSL1 mutants in CHO cells. CHO cells were transfected with plasmids carrying empty vector (EV), or a full-length rat ACSL1 cDNA either without mutation (NM), or with mutations to replace single amino acids A) at Ser278 with alanine (S278A) or aspartic acid (S278D), at Lys285 with alanine (K285A), glutamine (K285Q), or arginine (K285R), or B) at Lys676 witSh alanine (K676A), glutamine (K676Q), or arginine (K676R). Cell homogenates were collected 24h posttransfection. Total ACSL activity was measured using [1-14C]palmitate, data are means ± S.E. (n=3-6). * p<0.05, ** p<0.01. Western blotting with anti-ACSL1 antibody was performed with GAPDH as loading control, which has been repeated three times.