Sequence

Mouse Liver

Mouse Brown Adipose

Site  -5 -4 -3 -2 -1  0 +1 +2 +3 +4 +5

Fed [3]

4 hr fast [2]

20 hr fast [1]

4°C [1]

RT   [1]

None* [1]

Fed [2]

4 hr fast [0]

20 hr fast [3]

4°C [2]

RT [1]

T67    V   E    V T    G  T    T   E    G   V   R

x

 

 

 

 

 

 

 

 

x

 

S123   P  Y    E W   I   S   Y   K    Q   V   A

x

 

 

 

 

 

 

 

 

 

 

Y124   Y   E   W I    S Y     K Q     V   A   E

x

 

 

 

 

 

 

 

 

 

 

T219   V   E   N K    L  T     P   K   C    L   K I

x

 

 

 

 

 

x

 

 

 

x

S423   F   H   K I     Q S    S   L   G    G   K

 

 

 

 

 

 

 

 

x

 

(x)

S424  H   K   I   Q   S  S    L   G   G   K   V

 

 

 

 

 

 

 

 

x

 

(x)

Y500   V   E   E M    N Y    L    A   S   K   G

x

 

 

 

 

 

 

 

 

 

 

S503   M  N   Y  L    A S     K   G   E   G   E

x

x

 

 

 

 

 

 

 

 

 
x, Identified phosphorylations. Each “x” indicates that the modification was identified and multiple “x’s” indicate that the modification was identified by the corresponding number of replicates. For example, “xx” indicates that the modification was identified in 2 replicates.
[ ] Number of replicates.
( ) Ambiguous site (e.g. S423 or S424).
* Endogenous ACSL1 isolated from differentiated brown adipocytes
Abbreviations: RT, room temperature
Note: ACSL1 was immunopurified using gentle lysis buffer from liver crude mitiochondria, except for one of the 4 hr fasting liver samples (which was a lysate), and the brown adipose tissue lysates.
Table 4: Phosphorylation sites found in endogenous ACSL1from mouse liver lysate, liver crude mitochondria, or brown adipose tissue lysate.