Figure 3: Analysis of isoforms of α2u-globulin by 2D-PAGE and 2D-PAGE immunoblot. Panel A demonstrated the 2D-PAGE isoform distribution of α2u, it shows 10 spots with different pI ranges between 4.5 to 5. Panel B and C shows the separated 2D-PAGE isoforms were further confirmed with serine antiphosphorylation, which conclude that four spots were confirmed as phosphorylated.