Figure 1: Dnmt3a directly interacts with the DEAD box p68/Ddx5 RNA helicase. A. Schematic representation of GST-tagged Dnmt3a1 (Top), Dnmt3a2 (Middle) and His-tagged Ddx5 and Ddx5MUT (Bottom) proteins. Numbers indicate amino acid positions. B. Silver staining of an SDS-PAGE gel of pull-down reactions performed with GST-Dnmt3a2 (GST-3a2) and controls from mouse embryonic stem cell nuclear extracts (N.E). C. Western blot of pull-down reactions performed with GST, GSTDnmt3a1 and GST- Dnmt3a2 and mESC nuclear extracts. Detection was performed with Pab204 antibody. D. Western blot of pull-down reactions performed with GST, GST-Dnmt3a1 and GST- Dnmt3a2 and recombinant His-Ddx5 protein. Detection was with α-Xpress antibody.