Spot no.a Accession no.b Gene name Protein name Mr (E/T) (kDa)c pI (E/T)d Score Matched peptides Ratio averagee Regulation shAhR/mockf 1 Q9UL46 PSME2 Proteasome activator complex subunit 2 48.5/27.2 6.01/5.44 111 3 0.156 down 2 P14136 GFAP Glial fibrillary acidic protein 51.5/49.9 5.38/5.42 76 3 0.218 down 3 P10809 HSP60 60 kDa heat shock protein, mitochondrial 53.2/58.0 5.44/5.24 129 7 0.407 down 4 Q13322 GRB10 Growth factor receptor-bound protein 10 52.2/67.2 5.30/8.06 48 4 0.518 down A P30084 ECHS1 Enoyl-CoA hydratase, mitochondrial 27.5/28.3 6.15/5.88 153 3 1.24 up B P37837 TALDO Transaldolase 36.8/37.5 6.10/6.36 135 2 1.97 up C O95336 PGLS 6-phosphogluconolactonase 27.5/27.4 5.75/5.71 341 8 2.06 up D P61289 PSME3 Proteasome activator complex subunit 3 30.5/22.8 5.73/5.98 230 3 2.14 up E P09525 ANXA4 Annexin A4 31.5/35.8 5.74/5.85 50 3 2.19 up F P61981 YWHAG 14-3-3 protein gamma   28.2/28.3 4.60/4.80 125 8 2.44 up G P04792 HSP27 Heat shock protein beta-1 27.1/22.8 5.73/5.98 235 13 2.56 up H P04792 HSP27 Heat shock protein beta-1 26.1/22.8 5.31/5.98 171 3 6.29 up I P31946 YWHAB 14-3-3 protein beta/alpha 27.8/28.0 4.58/4.76 372 16 2.86 up J P09382 LGALS1 Galectin-1 14.5/14.6 4.95/5.34 172 8 3.23 up K P30566 ADSL Adenylosuccinate lyase 56.2/54.8 6.10/6.73 39 5 7.43 up L P60709 ACTB Actin, cytoplasmic 1 38.1/41.7 5.30/5.29 118 2 25.5 up M P60709 ACTB Actin, cytoplasmic 1 37.1/41.7 5.48/5.29 142 3 47.2 up aSpot number corresponds to the labels in Figure 2 and 3. b Accession number was from Swiss-Prot database. c Mr (E/T): experimental observation of apparent molecular weight in 2-DE/theoretical molecular weight calculated from protein sequence database. d pI (E/T): experimental observation of pI in 2-DE/theoretical pI calculated from protein sequence database. e The ratio average was calculated with six duplicates of shAhR cells divided by mock cells. f According to ratio average, smaller than 1 was as down-regulated and greater than 1 was as up-regulated.

Table 1: Identification of Differentially Expressed Proteins using Mass Spectrometry.