№ Protein name Swissprot accession number MW, Da % sequence coverage

20 slices

40 slices
1

Bile acyl-CoA synthetase

Q9Y2P5 75336 – 32±6
2

Liver carboxylesterase 1

P23141 62481 35±10 53±3
3

Chondroitin sulfate N-acetyl-galactosaminyl-transferase 1

Q8N6G5 62532 – 23
4

Dimethylaniline monooxy-genase [N-oxide-forming] 3

P31513 60033 – 34
5

Tyrosine-protein kinase Lyn

P07948 58537 23±2 33±3
6

Cytochrome P450 4A11

Q02928 59591 – 30±5
7

Cytochrome P450 4A22

Q5TCH4 59208 – 29±4
8

Cytochrome P450 1A2

P05177 58257 – 30±4
9

Cytochrome P450 3A4

P08684 57306 24±7 37±4
10

Cytochrome P450 2A13

Q16696 56665 33±4 39±3
11

Cytochrome P450 2E1

P05181 56812 – 39±2
12

Cytochrome P450 2A6

P11509 56509 49±3 48±4
13

Cytochrome P450 2B6

P20813 56242 – 25
14

Cytochrome P450 2C8

P10632 55825 40±1 –
15

Cytochrome P450 2C18

P33260 55711 34 35±4
16

Cytochrome P450 2F1

P24903 55466 – 34±2
17

Protein disulfide-isomerase

P07237 57116 58±4 44±4
18 Protein disulfide-isomerase A3 P30101 56747 40±6 47±4
19

Epoxide hydrolase 1

P07099 52915 41±1 56±10
20

Calreticulin

P27797 48112 37±2 61±2
21

Arylacetamide deacetylase

P22760 45719 – 35±4
22

Lactosylceramide 4-alpha-galactosyltransferase

Q9NPC4 40473 – 45
23

Heparan sulfate glucosamine 3-O-sulfotransferase 5

Q8IZT8 40383   – 35
24

Phosphatidylinositol N-acetylglu-cosaminyltransferase subunit A

 P37287 54127   – 32
25

Phosphatidylinositol-5-phosphate 4-kinase type-2 beta

P78356 47348   – 26±4
26

Serine incorporator 1

Q9NRX5 50461 – 27±2
27

Ras-related protein Rab-21

Q9UL25 24332 41 43
28

78 kDa glucose-regulated protein

P11021 72288  43 –
29

Erlin-1

O75477 38926 – 42
30

Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase

Q02742 49767 –   31
31

Keratin, type II cytoskeletal 1

P04264 66099 – 31
32

Synaptotagmin-like protein 3

Q4VX76 68560 37 28
33

Integrin beta-2

P05107 84726 20 –
34

Mitofusin-1

Q8IWA4 84047 – 25
35

ATP synthase subunit alpha, mitochondrial

P25705 59751 36±2 38
36

ATP synthase subunit beta, mitochondrial

P06576 56560 47±3 36±3
37

GRAM domain-containing protein 1C

Q8IYS0 76035 23 –
38

Transmembrane protein C3orf1

 Q9NPL8 33317 19 –
39

Tyrosine-protein kinase STYK1

Q6J9G0 47516 27±3 –
40 FERM domain-containing protein 3 A2A2Y4 65023 27±2 –
41

Cytochrome b-c1 complex subunit 2, mitochondrial

 P22695 48443 41 37
42

Long-chain fatty acid transport protein 4

P33121 77893 22 –
43 X-ray repair cross-complementing protein 5 P13010 82652   – 27
44 Amine oxidase [flavin-containing] A P21397 59644 – 35±3
45 Amine oxidase [flavin-containing] B P27338 58725 – 43±5
46 ADP-ribosil cyclase 1 P28907 34306 27 43
47 ADP/ATP translocase 4 Q9H0C2 34999 – 33
48 Trifunctional enzyme subunit beta, mitochondrial P55084 51262   32 36±4
49 Electron transfer flavoprotein -ubiquinone oxidoreductase Q16134 68452 – 24
50 Anthrax toxin receptor-like A6NF34 62864 – 23
51 Inositol 1,4,5-triphosphate receptor-interacting protein-like 2 Q3MIP1 58409   – 18
52

LEM domain-containing protein 2

Q8NC56 56940 – 25±2
53 Creatine kinase U-type, mitoch. P12532 47007 – 25
54

MOSC domain-containing protein 2, mitochondrial

Q969Z3 37999   – 36
55 RING finger and transmembrane domain-containing protein 1 Q5M7Z0 49677   – 20
56 Leucine-rich repeat transmembrane neuronal protein 3 Q86VH5 65854   – 18
57 Neuropilin and tolloid-like protein 2 Q8NC67 59354 – 36
58

MAGUK p55 subfamily member 6

Q9NZW5 61079 – 30±3
59 Transmembrane protease serine 11A Q6ZMR5 47538 – 20
60 Probable palmitoyltransferase ZDHHC23 Q8IYP9 45953   – 15
61

Tyrosine-protein kinase ZAP-70

P43403 69827 – 34
62

Phosphate carrier protein, mitochondrial

Q00325 40069   – 34±4
Table 1: Summary table of human liver membrane proteins identified in the region 35-75 kDa on the SDS-PAGE gel lane.