Figure 3a: Number of peptides detected in each FFE fraction. Peptides were separated using Mannitol, urea and thiourea based protocol using BD™ IEF Buffer pH 3-10 followed by identification by LC-MS/MS and database searching using an in-house Phenyx v 2.6 server (GeneBio, Switzerland). Only peptides with a p-score of 0.000001 and z-score of 5.5 are included.