Figure 3b: Protein distribution in each fraction. FFE-IEF workflow employing free flow electrophoresis for protein separation using 7 M urea, 2 M thiourea, 250 mM mannitol and carrier ampholytes (BD™ IEF Buffer pH 3-10). The pI range of each fraction was generated using in silico gel map generated using www.jvirgel.de.