ORF Protein GI II PsortB SOSUI LipoP FFE Fraction Corresponding pH Npun_F0166 hypothetical protein 1.065 40.21 Unk M SP I A5 5.59 Npun_F3800 acyltransferase 3 0.74 28.26 CM M   A4 3.04 Npun_F5190 hypothetical protein 0.64 18.59 Unk M SP I C4 4.36 Npun_F4347 major facilitator transporter 0.588 31.97 CM M   A7 6.85 Npun_R4363 ribosomal protein L13 0.588 31.97 Unk C   C6 6.45 Npun_F2140 glycolipid ABC exporter inner membrane subunit 0.348 25.72 CM M TMH F5 6.22 Npun_F2532 beta-Ig-H3/fasciclin 0.344 8.32 Unk C   A4 3.04 Npun_F4035 putative membrane-associated zinc metalloprotease 0.338 34.24 CM M   A6 6.32 Npun_F4291 microcompartments protein 0.317 28.05 Cyt C   A6 6.32

(GI: Grand average of hydropathicity (GRAVY) Index (http://www.expasy.ch/tools/ protparam.html), II (Insatiability Index(50)), PsortB(31), SOSUI(45). Abbreviations: M=membrane, C=Cytosolic, Unk=unknown, CM=Cytoplasmic Membrane, Cyt: Cytoplasmic

Table 1: Hydrophobic proteins detected in this study with information on their physico-chemical and biological characters such as Gravy Index Score, instability index and corresponding pH at which they were eluted in FFE wells. Biological characters showing their predicted sub-cellular location studied using PsortB and SOSUI algorithms. Proteins were also studied using LipoP algorithm for prediction of lipoproteins and discrimination of lipoprotein signal peptide and other signal peptides.