Figure 7: CXCL13-CXCR5 interaction leads to temporal activation of Src, ERK, FAK, and PI3K. FACE assays were performed to measure active and total Src, ERK, PI3K, and FAK in PC3 cell lysates. Cells were treated with CXCL13 (100 ng/ml) and were stimulated for 5 or 10 minutes. Untreated cells served as control. Experiments were performed in triplicates and results show ratios of active (phosphorylated) to total Src, ERK, PI3K, and FAK. Error bars represent ± standard error of means of three independent experiments.