(2b) Buffer optimization for leaf proteins of C. dactylon by increasing CHAPS Conc. 1-4%: C. dactylon proteins were solubilized in the rehydration buffer-II with the increase in CHAPS concentration 1-4%. IEF conditions were not changed.
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| Figure 2: (2a) Buffer optimization for leaf proteins of C. dactylon by
thiourea, DTT, and ampholyte pH 4/7 addition: C. dactylon leaf proteins were
solubilized in the rehydration buffer with the addition 7 M urea in combination
with 2 M thiourea, 1% ampholytes (pH 4/7) and replacing 2-merceptoethanol
with DTT while IEF conditions were kept the same.
(2b) Buffer optimization for leaf proteins of C. dactylon by increasing CHAPS Conc. 1-4%: C. dactylon proteins were solubilized in the rehydration buffer-II with the increase in CHAPS concentration 1-4%. IEF conditions were not changed. |
