(3b) Re-extracting solubilized proteins: The proteins were re-extracted from the rehydration buffer II by precipitating with 0.1M NH4OAC, washed with 90% ethanol and 80% acetone having 10mM DTT and re-solubilized in the rehydration buffer II.
|
| Figure 3: (3a) Buffer optimization for leaf proteins of C. dactylon with
rehydration buffer-II and 10mM DTT: Protein extraction was carried out from
C. dactylon with 10 mM DTT in acetone and followed by ethanol washings.
The rehydration buffer-II composition and IEF conditions were kept constant. (3b) Re-extracting solubilized proteins: The proteins were re-extracted from the rehydration buffer II by precipitating with 0.1M NH4OAC, washed with 90% ethanol and 80% acetone having 10mM DTT and re-solubilized in the rehydration buffer II. |
