Figure 2: Differential In-Gel Electrophoresis of total membrane proteins of carbapenem resistant strain RS307 of Acinetobacter baumannii in presence of iron relative to its absence. Total membrane proteins of RS307 in presence of iron (50 μg), its absence (50 μg) and pooled fraction (50 μg) were labelled using Cy3, Cy5 and Cy2 dye, respectively. Isoelectric focusing (IEF) was done using 13 cm, 4-7 pH range IPG strip and second dimension was done on 16×18 cm gel-electrophoresis. Gels were analyzed by Typhoon Imager. Upregulated proteins are seen as green while down regulated proteins are seen as red spots and unaltered proteins are seen as yellow.