Figure 5: Western blot analysis of HAP2 in various human materials. The numbers of samples were obtained from different sources and donors. Human serum samples (A); lanes: 1-3, normal serum samples; 4-9, lung cancer serum samples. Human lung tissues (B); lanes: 1-4, normal lung tissues; 5-8, lung cancer tissues. Lung cancer cell lines (C); lanes: 1, NCI-H838 adenocarcinoma cells; 2, SW900 squamous cell carcinoma cells; 3, NCI-H661 large cell carcinoma cells; 4, lung cancer serum samples (positive control); 5, HepG2 hepatocellular carcinoma cells. Cultured cell medium samples (D); lanes: 1, cultured medium of adenocarcinoma cells containing 10% fetal bovine serum (FBS); 2, cultured medium containing 5% FBS; 3, serum free medium; 4, lung cancer serum sample (positive control). Western blot analysis of co-immunoprecipitated haptoglobin in normal and lung cancer serum samples (E); lanes: 1, normal serum; 2, lung cancer serum; 3, immunoprecipitated haptoglobin in normal serum; 4, immunoprecipitated haptoglobin in lung cancer serum.