Figure 2: Hierarchical clustering of the 401 differentially expressed protein spots from the Base Set. The spot maps are displayed in rows and the protein spots by column. Differential expression is shown by color, with the red spots being up-regulated and the green being down-regulated relative to the pooled standard. The clustering shows that spot maps are grouped first by the replicate samples of each strain (KIM5 D27, India, NYC, and PEXU2), then by the growth condition (0 mM and 4 mM calcium chloride), and finally between strains, suggesting proteomic spot maps show more differences between strains than they do for different calcium growth conditions.