Figure 4: Hierarchical clustering of the 49 differential protein spots selected using the pattern-based marker selections method. The spot maps are displayed in columns and the protein spots by rows. Differential expression is shown by color, with the red boxes being up-regulated and the green being down-regulated relative to the pooled standard. The clustering shows that spot maps are grouped first by the replicates of eachstrain (KIM5 D27, India, NYC and PEXU2), then by the growth condition (0 mM and 4 mM calcium chloride), and finally between different strains. This hierarchy was the same when using the entire Base Set (see Figure 2).