Figure 2: Cellular localization of Mediator-HaloTag fusions and complex isolations. A) Confocal cellular images of HEK293T cells expressing HaloTag- MED12, HaloTag-MED13, HaloTag-MED13L, HaloTag-CDK8, HaloTag- CDK19, and HaloTag-MED26. All are labelled covalently with red TMR fluorescent HaloTag ligand and shown unclear localization. The far right panel of each shows the TMR staining, the middle panel shows the bright field image of the cells, and the far left panel is both the respective bright field with the TMR staining. B) Silver stained SDS gels of proteins eluted from pulldowns of HaloTag-MED12 (Panel 1), HaloTag-MED12L (Panel 2), HaloTag-MED13 (Panel 3), HaloTag-MED13L (Panel 4), HaloTag-CDK8 (Panel 5), HaloTag- CDK19 (Panel 6), HaloTag-Cyclin C (Panel 7), HaloTag-MED26 (Panel 8), and HaloTag alone control (Panel 9) in HEK293T cells with respective molecular weight (kDa) marker numbers indicated.