Figure 1: Time course evaluation of sample preparation methods. Plots illustrating the time- and denaturant-dependent production of target peptides from membrane fractions for (a) OATP1B1, surrogate-1, (b) OATP1B3, surrogate-1, and (c) OATP2B1, surrogate-1. All samples were processed from the same initial pellet for each transfected cell line to rule out variability in starting material. Membrane fractions were initially denatured in 25 mM ABC alone or 25 mM ABC containing 7 M Gdn, 0.2% PMAX, or 10% DOC prior to alkylation, dilution, and digestion. Data represent the mean ± standard error of the mean (S.E.M.) for three transitions measured for each peptide by LC-MS/MS at each time point.