18FDG-PET/CT Definition of Clinical Target Volume for Robotic Stereotactic Body Radiosurgery Treatment of Metastatic Gynecologic Malignancies

The objective of the current article was to evaluate 2-[18F]fluoro-2-deoxy-D-glucose (18F-FDG) as measured by positron emission tomography for delineation of abdominopelvic gross tumor volumes (GTV) for stereotactic body radiosurgery treatment (SBRT) of metastatic gynecologic cancers. A retrospective review of SBRT was conducted in 27 women with stage IV gynecologic cancers recurring in para-aortic lymph nodes. Robotic SBRT involved 2400 cGy in 3 consecutive 800 cGy daily fractions prescribed to a 3.0 mm expanded planning tumor volume (PTV) defined by both CT-based and 18F-FDG-based GTVs. In this study, 18F-FDG-based GTVs led to significantly larger PTVs in all 27 women, than if they had been based on CT GTVs alone (P < 0.001). Enlarged PTVs may have resulted from the breathing-induced target motion during the time of 18F-FDG image acquisition smearing 18F-FDG signal over a greater anatomic dimension. Ultimately, SBRT-target local control, based on the RECIST 1.1 criteria, was 96% (26 of 27), and associated with minor reversible toxicity. The use of 18F-FDG to define SBRT target volumes warrants further interrogation in SBRT clinical trials. J o u r n a l o f N uc lea r M edicine & Riatio n T h e r a p y ISSN: 2155-9619 Journal of Nuclear Medicine & Radiation Therapy Citation: Kunos CA, Debernardo R, Fabien MSJ, Dobbins DC, Zhang Y, et al. (2011) 18FDG-PET/CT Definition of Clinical Target Volume for Robotic Stereotactic Body Radiosurgery Treatment of Metastatic Gynecologic Malignancies. J Nucl Med Radiat Ther S4:001. doi:10.4172/21559619.S4-001


Introduction
This article explores the utility of 2-[ 18 F] fluoro-2-deoxy-D-glucose ( 18 F-FDG) for delineation of stereotactic body radiosurgery cancer targets when treating abdominopelvic sites of metastatic gynecologic cancers. Many radiation oncologists have adopted either side-by-side comparisons, image overlays, or direct fusion of positron emission tomography (PET) and computed tomography (CT) images for the purpose of radiation treatment planning. The radioactive tracer 18 F-FDG, a sugar analogue taken up and sequestered in cells by the intracellular enzyme hexokinase, helps spot or make more clear cancer targets [1][2][3][4][5]. Treating radiation oncologists often regard 18 F-FDG uptake by cancer cells as a marker of cancer cell viability, and subsequently focus radiation dose on ' 18 F-FDG-viable' cancer targets in an effort to improve therapeutic gain. Physician contours for cancer targets, and thus, the intended radiotherapeutic clinical target volumes, are influenced by threshold settings and by respiration-driven motion recorded on fused 18 F-FDG PET and CT images [6]. As radiation therapy devices become more accurate and more precise in their delivery of radiation, accurate anatomic and metabolic delineation of cancer targets becomes imperative.
Stereotactic body radiosurgery has an emerging role in the management of persistent or recurrent gynecologic malignancies [7,8]. A frameless, robotic Cyberknife® stereotactic body radiosurgery system (Accuray, Sunnyvale, CA) is but one example of a radiation delivery system where sub-millimeter accuracy and precision of ablative radiation dose can be applied in abdominopelvic recurrences of gynecologic malignancies [9,10]. While early clinical success has been documented with this radiosurgical technique [11,12], contouring guidelines for the use of 18 F-FDG PET image overlays paired with anatomical CT images have not been fully explored. This retrospective study offers initial guidelines for radiosurgical target contouring using overlaid 18 F-FDG PET and CT images among women with abdominopelvic para-aortic lymph node recurrences of gynecologic cancers.

Methods and Materials Patients
Institutional review board approval was granted for study prior to review of stereotactic body radiosurgery datasets. All women provided informed consent for stereotactic body radiation. For this article, inclusion criteria were female sex, an age of 18 years or older, and a diagnosis of metastatic stage IV gynecological cancer occurring in an abdominopelvic para-aortic lymph node site. In our stereotactic body radiosurgery program, 27 women met these criteria at retrospective study selection among 74 women treated between 2007 and 2011 [ Figure 1]. Table 1 lists patient demographics, gynecologic cancer type, number of sites to be treated by radiosurgery, and administered prior therapies.

Stereotactic body radiosurgery 18 F-FDG PET and CT simulation
Women either had three or more 1.6 × 3 mm soft tissue gold seed fiducials implanted within 4 cm to 6 cm of the radiosurgical targets under anesthesia or had bony landmarks of the spine identified for The objective of the current article was to evaluate 2-[ 18 F]fluoro-2-deoxy-D-glucose ( 18 F-FDG) as measured by positron emission tomography for delineation of abdominopelvic gross tumor volumes (GTV) for stereotactic body radiosurgery treatment (SBRT) of metastatic gynecologic cancers. A retrospective review of SBRT was conducted in 27 women with stage IV gynecologic cancers recurring in para-aortic lymph nodes. Robotic SBRT involved 2400 cGy in 3 consecutive 800 cGy daily fractions prescribed to a 3.0 mm expanded planning tumor volume (PTV) defined by both CT-based and 18 F-FDG-based GTVs. In this study, 18 F-FDG-based GTVs led to significantly larger PTVs in all 27 women, than if they had been based on CT GTVs alone (P < 0.001). Enlarged PTVs may have resulted from the breathing-induced target motion during the time of 18 F-FDG image acquisition smearing 18 F-FDG signal over a greater anatomic dimension. Ultimately, SBRT-target local control, based on the RECIST 1.1 criteria, was 96% (26 of 27), and associated with minor reversible toxicity. The use of 18 F-FDG to define SBRT target volumes warrants further interrogation in SBRT clinical trials.

Nuclear Medicine & Radiation Therapy
radiosurgical targeting [8]. Women were scanned on a flat tabletop either with two-pin knee sponge tabletop registry or with evacuated vacuum-bag immobilization for reproducible body alignment. For target motion due to quiet breathing, women wore a firmly-conforming vest with light-emitting diodes tracked by in-room motion-detecting cameras. If respiration was anticipated clinically to move the target, the Synchrony Motion Tracking system (MTS) was selected and was utilized during delivery of radiation.
Women rested comfortably in a head-first treatment position with their arms across their chest for a mid-thorax to mid-thigh non-contrasted contiguous helical CT high-resolution scan (1 to 1 pitch, voxel 0.98 x 0.98 x 1.0 mm, voltage 120 kVp, 450 mAs; 64-slice SOMATOM Sensation scanner, Siemens Medical Solutions). CT images were acquired during quiet breathing. Afterwards, women had Contemporaneously, low dose non contrast CT images were acquired over the torso (5mm intervals, voltage 120 kVp, 60-90 mAs with dose modulation). 18 F-FDG PET images were reconstructed with and without CT attenuation correction (AC) following institutional protocol [13]. Importation, digital overlay, and co-registration of high resolution CT and AC 18 F-FDG PET images were done by a certified medical physicist in the MultiPlan 3.5.2 Treatment Planning System (Accuray).

Clinical target definitions
CT-based abdominopelvic gross tumor volumes (CT-GTV) was contoured with abdomen window settings (1,200 and 0), and then, agreed by both a treating radiation oncologist and gynecologic oncologist. 18 F-FDG PET gross tumor volume (PET-GTV), with a user-defined PET setting threshold of about 40% of the standard uptake value maximum selected on the basis of single standardized uptake value threshold approaches in lung cancer [6], was delineated for each CT-GTV image using co-registered axial, coronal, and sagittal images. Ellipsoid PET-GTV regions of interest were agreed upon after independent review by both the treating radiation oncologist and gynecologic oncologist. Inverse treatment planning was done by a certified medical dosimetrist utilizing typically a 3.0 mm margin encompassing the PET-GTV to create a radiosurgical planning tumor volume (PTV) [12]. Nearby normal tissue structures such as the small bowel, rectum, bladder, liver, kidneys, lungs, bilateral proximal femurs, vagina, and sacral nerve roots were contoured by the radiation oncologist, medical physicist, or certified medical dosimetrist. For this article, volume calculations in cubic centimeters for CT-GTV, PET-GTV and PTV were determined by dosimetric convention. Longest sum diameter of lymph node short axis for radiosurgical targets were recorded following guidelines of RECIST version 1.1 (complete response [CR] = reduction of target lymph node < 10 mm; partial response [PR] = persistence of lymph node(s) equating to non-CR and non-progressive disease). Descriptive and graphical statistics were computed using statistical software (SPSS 18.0, Chicago, IL).

Treatment Planning, Radiation Delivery, and Follow-up
Based on prior clinical experience [11,12], a radiation prescription dose of 800 cGy per fraction totaling 2400 cGy (prescribed to the 70% isodose line) was employed for three consecutive daily fractions of stereotactic body radiosurgery. The Cyberknife 6 MV SBRT linear accelerator was collimated either by using one of 12 fixed tungsten circular collimators or by using a tungsten-copper alloy segmented hexagon IRIS collimator [14]. Treatment parameters are listed in Table 2. Women were seen at one and then every three months after therapy for toxicity assessments. Common toxicity criteria for adverse events (version 4.0) were retrospectively abstracted from self-reported treatment-related toxicity at one-and three-month visits. Restaging CT imaging was obtained at one-and three-months after therapy, and then at investigator discretion.

Results
27 women had stage IV gynecologic cancers with individual or a single conglomerate of multiple para-aortic lymph node metastases measurable on CT and 18 F-FDG PET images. Median clinical follow-up was 8 months (25%-75% quartile: 5 months -13 months). Eight (30%) of 27 women have died from non-radiosurgical target gynecologic cancer disease progression during clinical follow-up.

Exclude SBRT-treated non-para-aortic nodal disease (n = 44)
Multiple individual radiation beams were used to deliver stereotactic body radiation, with beams converging on single or multiple closelyassociated clinical abdominopelvic cancer targets ( Figure 3, Table 2). To provide coverage of the PTV with 2400 cGy, a median isodose line contour of 70% (25%-75% quartile: 70% -75%) was used as the prescription line. 1-and 3-month radiosurgical target response rates (CR + PR) were 96% (26 of 27) and 96% (26 of 27), respectively. One patient has had stable para-aortic lymph node short-axis dimension throughout her follow-up. Post-therapy 18 F-FDG PET images for standard uptake value response were not acquired. Non-target disease progression occurred in 13 (48%) of the 27 women. Within 30 days after radiosurgery treatment, eight (30%) had self-reported grade 2 fatigue after radiosurgery and two (7%) had a grade 2 increase of 4 -6 stools per day over baseline.

Discussion
This retrospective study showed that 18 F-FDG PET-based contours were significantly larger than CT-based contours among women with metastatic abdominopelvic gynecologic cancer. Irradiated planning tumor volumes were sterilized at a rate of 96% in this series with minimal morbidity.
In this series of women with recurrent gynecologic cancers in paraaortic lymph nodes, both CT-based and 18 F-FDG PET-based contours contributed data used to generate the radiosurgical planning tumor volume. Use of both imaging sets was chosen by the investigators based on the hypothesis that 18 F-FDG PET images acquired during quiet breathing in part may reflect the relative inspiratory-expiratory paths of abdominopelvic targets during respiration. Given the overall accuracy and sub-millimeter precision of robotic stereotactic body radiosurgery, the investigators desired to have as much information regarding target movement during radiosurgical planning for ablative dose delivery. It is conceivable that abdominopelvic targets not fixed to the abdominal cavity could have moved and could have resided outside the treatment path of such narrowly-collimated radiation beams used in robotic stereotactic body radiosurgery. While the investigators recognize that the Cyberknife's capability for monitoring and correcting for intrafraction target motion lowers the hazard of missing a radiosurgical target, the extent of tumor motion may not be captured fully on static CT-based imaging resulting in an actual planning tumor volume that may not entirely encompass moving targets in real-time. The investigators speculated that overall respiratory motion and time spent by abdominopelvic tumor targets in different portions of the breathing cycle contribute to 18 F-FDG PET uptake fluctuations, as others have [6]. Certainly, an acknowledged limitation of 18 F-FDG PET-based contouring for radiation planning is the subjective nature of user-defined image threshold settings. Although a 40% threshold was used consistently in our study, other threshold values could just as well serve to adequately delineate tumor volumes. In previous study of stereotactic body radiosurgery for recurrent vulvar cancers without 18 F-FDG PET-based contouring [12], ablative radiation dose initially sterilized clinical and CT identified cancer targets in all three women studied. And yet, follow-up revealed disease progression in immediately adjacent tissue not targeted by radiosurgery in all three women. By contrast, the high therapeutic response rate (96%) presented here on larger 18 F-FDG PET-based and CT-based contours attests to greater accounting of both nearby occult disease and target motion due to respiration. Notwithstanding these results, further research is needed before drawing broad conclusions regarding 18 F-FDG PET and a percentage threshold method for radiation planning.
This article has limitations of small sample size; user-defined 18 F-FDG PET threshold settings, potential investigator bias in   retrospective assignment of toxicity criteria; and limited overall followup. Accrual to a 50 patient prospective robotic stereotactic body radiosurgery trial for women with metastatic gynecologic malignancies using more rigorous 18 F-FDG PET guidelines has been completed in an effort to report safety, tolerability, and outcomes measures. Mature results are eagerly awaited [15].
As stereotactic body radiosurgery for abdominopelvic sites of metastatic gynecologic cancers becomes more mainstream, accumulated 18 F-FDG PET and CT data will better guide treating physician target contouring. Future research directions by our group will study better radiosurgical target delineation, as modified by the extent of respiratory motion, by four-dimensional (4D)-CT scanners or by single 18 F-FDG PET-magnetic resonance imaging (PET/MRI) scanners.