Table 1

QC Level	Predicted concentration^a (ng/ mL)		Mean accuracy^e (%)	Intra-day repeatability (%RSD) (n = 5)			Inter-day repeatability (%RSD) (n Meanb ± = 30)
QC Level	Meanb ±SD^c	%RSD^d	Mean accuracy^e (%)	Day-1	Day-2	Day-3	Inter-day repeatability (%RSD) (n Meanb ± = 30)
LQC (250 ng/mL)	258.14 ± 10.62	4.11	3.25	4.04 3.78	4.10 3.97	4.06 4.04	4.02
MQC (750 ng/mL)	740.85 ± 23.14	3.12	-1.22	3.24 3.08	3.17 2.97	3.26 3.14	3.17
HQC (3000 ng/ mL)	3037.75 ± 73.68	2.42	1.25	2.54 2.78	3.17 2.97	2.86 3.04	2.75

^aEach value is mean of ten independent determinations (n = 10).
^bPredicted concentration of RLZ was calculated from linear regression equation.
^cStandard deviation.
^dPercentage relative standard deviation.
^eAccuracy is given in relative error % = [100 × (predicted concentration – nominal concentration)/nominal concentration.)].

Table 1: Results of accuracy and intermediate precision studies for the proposed method in rat plasma.