Step

Collected Volume

Total Activity(IU)

Total Protein(mg)

Specific activity(IU ml)

Purification (Fold)

YIELD( %)

Crude `Extract

 450 87871.5 4339.82 20.24

100

(NH4)2SO4

 300 72726 316.985 229.43 11.33 82.76

DEAE cellulose column

 230 63567.4 67.705 938.88 46.37 72.34

Sephadex G200 column

 135 49889.25 23.549 2118.52 104.63 56.77

Prep Cell

 95 70927 14.086 5035.28 248.68 80.71
Table 2: Summary of steps employed in purification of L-asparaginase from MS1.