Figure 1: Protein profiles of dialyzed epididymal lystates of rabbit applied onto a CMC column. Elution pattern of ≤ 30 kDa proteins, concentrated by ultrafiltation (a). Unbound fractions were washed with 0.2 M sodium acetate (1 ml fractions) until ~110 ml of buffer passed through the column. Bound fractions were eluted with 0.2 M ammonium acetate. (b) Antimicrobial activity of fractions were tested against E. coli and S. aureus by radial diffusion assay and expressed as area of inhibition (mm2).