Figure 2: Peak-II fractions obtained on cation-exchange chromatography separated on a Vydac C8 RP-HPLC column equilibrated with 0.1% (v/v) TFA / water. (a) Elution (1 ml/min) was performed according to the procedure described in the material and methods section. (b)The active pooled fractions (peaks-1 to 5) showed four protein bands at 15, 17, 22 and 27 kDa region on SDS-PAGE (Lane 1= uninduced and 2= induced pooled fractions Peaks 1-5 demonstrated antimicrobial activity as tested by radial diffusion assay against E. coli and S. aureus (c).Protein obtained from several runs were pooled peak wise and antimicrobial activity was shown against E. coli and S. aureus.