Figure 3: 2D-PAGE map of rabbit epididymal RP-HPLC purified pooled fractions (peaks 1-5) (a). Eighteen spots were observed. Peak-II pooled fractions obtained on cation exchange chromatography labelled with biotin and allowed to interact with HIV antigens on nitrocellulose blots and compared with standard HIV blot (b-1) and positive control (b-2). Biotin labeled proteins bound to HIV strip (b-3) were eluted as described in material and methods section and subjected to 2D-PAGE. Four spots were observed (c).