| Gene |
Thermocycler program |
Restriction enzyme |
Genotypes |
Restriction fragments |
| CASP8-D302H |
94°C for 1 min., 35 cycles of 94°C for 40 sec, 60°C for 40 sec, 7°C for 40 sec., then final extension at 72°C for 5 min. |
BstUI |
Wild genotype
Homotype
Heterotype |
132 bp
112+20 bp
132+112+20 bp |
| CASP8- 625 6N |
BfaI |
Wild genotype
Homotype
Heterotype |
171 bp
146+ 31 bp
171+146+31 bp |
| CASP10- 1522L |
94°C for 1 min., 30 cycles 94°C for 40 sec, 50°C for 40 sec, 72°C for 40 sec., then final extension step at 72°C for 5 min. |
SspI |
Wild genotype
Homotype
Heterotype |
122 bp
97+25 bp
122+97+25 bp |