Gene Thermocycler program Restriction enzyme Genotypes Restriction fragments
CASP8-D302H 94°C for 1 min., 35 cycles of 94°C for 40 sec, 60°C for 40 sec, 7°C for 40 sec., then final extension at 72°C for 5 min. BstUI Wild genotype
Homotype
Heterotype
132 bp
112+20 bp
132+112+20 bp
CASP8- 625 6N BfaI Wild genotype
Homotype
Heterotype
171 bp
146+ 31 bp
171+146+31 bp
CASP10- 1522L 94°C for 1 min., 30 cycles 94°C for 40 sec, 50°C for 40 sec, 72°C for 40 sec., then final extension step at 72°C for 5 min. SspI Wild genotype
Homotype
Heterotype
122 bp
97+25 bp
122+97+25 bp
Table 3: Genotyping of Caspase 8-D302H, Caspase 8-652 6 N ins/del and Caspase 10-1522L genes by PCR-RFLP assay.