Figure 1: Characterization of the SGLT1-WB antibody by Western blot analysis. Detection of FLAG-tagged SGTL1 transiently transfected into HEK293T cells in combination with control shRNA or SGLT1 shRNA, using A, an anti-FLAG primary antibody or B, SGLT1-WB. C, WB detection of immunoprecipitated flagged SGLT1 expressed in HEK293 cells using the SGLT1-WB antibody. D, SGLT1 expression in PC3, PC3-MM2, and LNCaP cells. E, Detection of endogenous SGLT1 of PC3 cells transfected with control shRNA or SGLT1 shRNA, using SGLT1-WB. Actin was used as the loading control.