Figure 1: Immunocytochemical analysiswas performed using cell blocks of JN-DSRCT and SKBR-3 cell lines. In this assay, SKBR-3, a breast cancer cell line, was used as a positive control for HER2, HER3, and EGFR expressions. HER2, HER3, and EGFR expressions were not observed in JN-DSRCT cell line (B, D, and F), though expressions of these proteins were seen in SKBR-3 cells (A, C, and E). This analysis confirmed FGFR4 overexpression in a JN-DSRCT cell line (G). FGFR4 was over expressed in one of the 2DSRCT clinical samples (H).