Method AVS (µg/mL) Er% RSD (%) %Recovery ± SD Taken Founda Initial rate 2.00 2.01 0.49 3.08 100.50 ± 0.32 4.00 4.06 1.48 2.52 101.50 ± 0.41 12.00 12.12 0.99 1.58 101.00 ± 0.36 16.00 16.14 0.86 0.92 100.87 ± 0.30 20.00 20.13 0.64 0.73 100.65 ± 0.29 Fixed time 2.00 2.02 0.99 2.85 101.00 ± 0.30 4.00 4.05 1.23 2.02 101.25 ± 0.27 12.00 12.09 0.74 1.28 100.75 ± 0.33 16.00 16.08 0.50 0.85 100.50 ± 0.28 20.00 20.19 0.94 0.64 100.95 ± 0.21 aAverage of six determinations.

Table 5: Accuracy and precision for the determination of AVS in bulk powder by the proposed initial rate and fixed time methods.