| S. No. |
Type of Media |
HPLC Column |
HPLC Mobile Phase |
Mass Analyzer |
Chromatographic conditions |
Reference |
| 1. |
Rat plasma and bile |
AM-312 (ODS, 150 × 6 mm, 5μm) |
Water-acetonitrile-trifluoroacetic acid (70 : 30: 0.1v/v) |
Finnigan MAT (QqQ) |
Flow rate: 1.0 mL.min-1 Temperature: ambient |
[52] |
| 2. |
Rat plasma |
ACE C18 column (50 × 4.6 mm, 5 μm) |
10 mM ammonium acetate:acetonitrile (20:80, v/v) |
MDS Sciex API 4000 (Q) |
Flow rate: 0.5 mL.min-1 Temperature: 40 ± 2°C Detection: PDA (254 nm) |
[54] |
| 3. |
Human plasma |
Synergy Polar RP (150 × 2 mm, 4μm) |
Solvent A: 0.1 formic acid (v/v) with 1 mmol/L ammonium formate Solvent B: acetonitrile: 0.1 formic acid 95:5 (v/v) with 1 mmol/L ammonium formate |
API 365 (QqQ) |
Flow rate: 0.25 mL.min-1 Temperature: 40°C |
[55] |
| 4. |
Rabbit eyes |
Capcell Pak (4.6 × 250 mm, 5 μm) |
Acetonitrile and water (0.1 formic acid) |
6410 (QqQ) |
Flow rate: 1.0 mL.min-1 Temperature: room |
[57] |
| 5. |
Stability studies |
Luna C18 (150 × 4.6 mm, 5 μm) |
Acetonitrile and potassium dihydrogen orthophosphate buffer (pH 2.8; 0.01M) in a gradient mode |
MicrOTOF-Q (TOF) |
Flow rate: 1.0 mL.min-1 Temperature: room Detection: PDA (254 nm) |
[58] |
| 6. |
Impurity profile |
Kromasil cyano column (250 × 4.6 mm, 5 μm) |
Water adjusted to pH 3.0 with TFA (A) and acetonitrile (B) in a gradient mode |
PolarisQ (IT) |
Flow rate: 1.0 mL.min-1 Temperature: room Detection: PDA (210 nm) |
[59] |
| 7. |
Human plasma |
BEH C18, 1.7 μm column (analysis performed with Acquity UPLC) |
0.1(v/v) formic acid in Distilled water and 0.1(v/v) formic acid in acetonitrile (60: 40, v/v) |
Micromass Quattro Premier XE |
Flow rate: 0.4 mL.min-1 Temperature: room |
[60] |
| 8. |
Rat plasma |
Zorbax SB-C18 (150 × 4.6 mm) with a C18 guard column (4 × 3.0 mm, Phenomenex) |
Methanol and 5 mM ammonium acetate (50:50 v/v) |
MDS Sciex API 4000 |
Flow rate: 0.6 mL.min-1 Temperature: room Detection: PDA (254 nm) |
[61] |
Q: Single quadrupole, QqQ: Triple quadrupole, IT: Ion trap, TOF: Time of flight, Q-TOF: quadrupole-time of flight