| Phenomena |
Procedures |
| Aggregation |
SDS-PAGE, SEC-HPLC. light scattering |
| Deamidation, Oxidation |
Peptide map, HPLC, IEF, MS |
| Proteolytic Cleavage |
Peptide map, SDS-PAGE, HPLC, IEF, MS |
| Amino Acid Substitutions |
AAA, Peptide map, MS, protein sequence, CE |
| Translation Mutations |
Peptide map, HPLC, IEF, MS, CE |
| Host Cell Proteins |
SDS-PAGE, Western blot, ELISA |
| Media Components |
SDS-PAGE, Western blot, HPLC, ELISA |
| Nucleic Acids |
DNA hybridization, UV, Protein binding |
| Affinity Antibodies |
SDS-PAGE, Western blot, ELISA |
| Proteases/Nucleases |
HPLC, Western blot, ELISA |
| Leachates/Extractables |
HPLC, MS, GC, gravimetric analysis |
| Process Residuals |
Karl Fisher moisture, GC, ion chromatography |
|