Michaelis-Menten model Substrate inhibition model Parameters XL_Kinetics Graphpad Prism SigmaPlot XL_Kinetics Graphpad Prism SigmaPlot Km 0.5268 ± 0.0433 0.5268 ± 0.0433 0.5268 ± 0.0433 4.5783 ± 0.6399 4.5784 ± 0.6399 4.5783 ± 0.6399 Vmax 4.4013 ± 0.1113 4.4013 ± 0.1113 4.4013 ± 0.1113 2.0336 ± 0.1517 2.0336 ± 0.1517 2.0336 ± 0.1517 Ks       70.9095 ± 17.3603 70.9093 ± 17.3602 70.9095 ± 17.3603 Diagnostics             R2 0.9955 0.9955 0.9955 0.9958 0.9958 0.9958 SS 0.0632 0.0632 0.0632 0.0065 0.0065 0.0065 SE 0.1026 0.1026 0.1026 0.0362 0.0362 0.0362 AIC* -18.0978     -34.2414     SC* -17.9389     -34.0031     *AIC and SC are not provided in Graphpad Prism and SigmaPlot.

Table 2: Detailed comparison of XL_Kinetics results with those of Graphpad Prism 5.0 and SigmaPlot 11.0 (Michaelis-Menten and substrate inhibition models)