Competitive inhibition model Noncompetitive inhibition model Uncompetitive inhibition model Mixed-type inhibition model Parameters XL_Kinetics Graphpad Prism XL_Kinetics Graphpad Prism XL_Kinetics Graphpad Prism XL_Kinetics Graphpad Prism Km 3.4968 ± 0.8099 3.4968 ± 0.8099 5.5511 ± 0.5512 5.5511 ± 0.5512 7.2424 ± 1.1466 7.2424 ± 1.1466 4.8663 ± 0.6438 4.8664 ± 0.6438 Vmax 1.6238 ± 0.0978 1.6238 ± 0.0978 1.8835 ± 0.0671 1.8835 ± 0.0671 2.0026 ± 0.1251 2.0026 ± 0.1251 1.8194 ± 0.0739 1.8194 ± 0.0739 Ki 0.1657 ± 0.0394 0.1657 ± 0.0394 0.8417 ± 0.0701 0.8417 ± 0.0701 0.5942 ± 0.0817 0.5942 ± 0.0817 0.5268 ± 0.1466 0.5269 ± 0.1467 Alpha (a)             1.9835 ± 0.8329 1.9834 ± 0.8329 Diagnostics                 R2 0.9302 0.9302 0.9302 0.9767 0.9492 0.9492 0.9792 0.9792 SS 0.2825 0.2825 0.0944 0.0944 0.2053 0.2053 0.0843 0.0843 SE 0.1160 0.1160 0.0670 0.0670 0.0989 0.0989 0.0649 0.0649 AIC* -24.3368   -50.6453   -31.9966   -51.3623   SC* -20.8026   -47.1111   -28.4624   -46.6501   *AIC and SC are not provided in Graphpad Prism.

Table 5: Detailed comparison of XL_Kinetics results with those of Graphpad Prism 5.0 (inhibition modules)