Items Membrane Chromatography Adsorber (70 ml) Large Scale Packed Bed Chromatography (40 L) Pre-equilibration NA 3 CV = 120 L Equilibration 10 DV =3.5 L 5 CV = 200 L Wash 30 DV = 10.5 L 3 CV= 120 L Regeneration NA 3 CV = 120 L Storage NA 3 CV = 120 L Total 14l 680 L

Table 1: Comparison of buffer usage for large scale packed bed chromatography and membrane. Chromatography Adsorber.