SNP ID (this study) SNP ID (NCBI rs#) Gene Alleles MAFa Location Amino Acid Change Chromosome Position (bp) Genotyping Method M1 rs1799971 OPRM1 A118G 0.13 (G) Exon 1 Asn40Asp Chr06: 154402490 PCR-RFLP M2 rs511435 OPRM1 A/G 0.21 (A) Intron 1 - Chr06: 154410240 TaqMan M3 rs524731 OPRM1 A/C 0.20 (A) Intron 1 - Chr06: 154416785 TaqMan M4 rs3823010 OPRM1 A/G 0.15 (A) Intron 1 - Chr06: 154420845 TaqMan M5 rs495491 OPRM1 C/T 0.24 (C) Intron 1 - Chr06: 154424235 TaqMan M6 rs1381376 OPRM1 A/G 0.15 (A) Intron 1 - Chr06: 154434951 TaqMan M7 rs3778156 OPRM1 A/G 0.15 (G) Intron 1 - Chr06: 154446006 TaqMan M8 rs2075572 OPRM1 C/G 0.43 (G) Intron 2 - Chr06: 154453697 TaqMan M9 rs548646 OPRM1 C/T 0.34 (T) Intron 3 - Chr06: 154459840 TaqMan M10 rs9322447 OPRM1 A/G 0.48 (A) Intron 3 - Chr06: 154466013 TaqMan M11 rs609148 OPRM1 C/T 0.25 (T) Intron 3 - Chr06: 154472707 TaqMan M12 rs648893 OPRM1 C/T 0.25 (C) Intron 3 - Chr06: 154480321 TaqMan M13 rs671531 OPRM1 A/G 0.35 (A) downstream   Chr06: 154482434 TaqMan D1 rs569356 OPRD1 C/T 0.12 (C) upstream   Chr01: 29009273 PCR-RFLP D2 rs1042114 OPRD1 G80T 0.13 (G) exon 1 Cys27Phe Chr01: 29011562 PCR-RFLP D3 rs678849 OPRD1 C/T 0.47 (C) intron 1 - Chr01: 29017775 PCR-RFLP D4 rs2236857 OPRD1 A/G 0.30 (G) intron 1 - Chr01: 29034196 TaqMan D5 rs2236855 OPRD1 G/T 0.29 (T) intron 1 - Chr01: 29034586 TaqMan D6 rs2298896 OPRD1 A/C 0.37 (C) intron 1 - Chr01: 29038725 TaqMan D7 rs421300 OPRD1 C/T 0.38 (C) intron 1 - Chr01: 29042180 TaqMan D8 rs529520 OPRD1 G/T 0.50 (T) intron 1 - Chr01: 29047533 TaqMan D9 rs12749204 OPRD1 A/G 0.21 (G) intron 1 - Chr01: 29048800 TaqMan D10 rs2234918 OPRD1 C921T 0.44 (C) exon 3 Gly307Gly Chr01: 29062184 PCR-RFLP D11 rs204076 OPRD1 A/T 0.34 (A) downstream   Chr01: 29062977 TaqMan K1 rs12675595 OPRK1 A/G 0.09 (A) upstream   Chr08: 54330478 TaqMan K2 rs1051660 OPRK1 G36T 0.10 (T) exon 1 Pro12Pro Chr08: 54326115 TaqMan K3 rs6985606 OPRK1 C/T 0.46 (T) intron 1 - Chr08: 54323669 TaqMan K4 rs997917 OPRK1 C/T 0.34 (C) intron 1 - Chr08: 54314931 TaqMan K5 rs702764 OPRK1 C843T 0.12 (C) exon 3 Ala281Ala Chr08: 54304710 TaqMan K6 rs963549 OPRK1 C/T 0.14 (T) exon 3 (UTR)   Chr08: 54304377 PCR-RFLP K7 rs7820807 OPRK1 C/T 0.12 (C) downstream   Chr08: 54301414 TaqMan aMarker minor allele frequency (MAF) in European American (EA) healthy control subjects

Table 1: Information and genotyping methods for OPRM1, OPRD1and OPRK1 Single Nucleotide Polymorphisms (SNPs).