| Components |
Volume mg/ 25 ml |
| NaCl |
167.5 mg/ml |
| KCl |
5.8 mg |
| NaHCO3 |
55 mg |
| BSA (Fatty Acid Free) |
75 mg |
| Hemi-Ca Lactate |
13.8 mg |
| Pyruvate |
1 mg |
| L-Glutamine |
3.8 mg |
| Basal Medium Eagle (BMA) (50X Stock) amino acids |
500 µl |
| Minimum Essential medium (MEM) amini acids (100X stock) |
250 µl |
| Gentamycin |
12.5 µl |
| EGF |
25 µl |
| ITS |
5 µl |
|
