Figure 3: Long-term effects of chronic prenatal ethanol exposure on signaling through Akt and GSK-3β. P30 cerebellar protein homogenates were used to measure (A) Akt, (B) GSK-3β, (C) pS473-Akt, and (D) pS9-GSK3β immunoreactivity by multiplex ELISA. (E,F) Phospho-/total protein ratios were calculated. Inter-group comparisons were made using Student t-tests. Significant differences are indicated within the panels. N=12/group with each sample contributed from a different litter.