Figure 4: The effect of exercise on GLUT-4 protein expression and translocation in the gastrocnemius muscle. (A) Representative immunoblotting results for GLUT-4 protein in cytosolic and membrane fractions isolated from the gastrocnemius muscle are depicted in the upper panel. The lower panel shows a histogram of GLUT-4 protein levels as assessed from the immunoblots by densitometry. (B) The level of GLUT-4 translocation was calculated by assessing the difference in GLUT-4 protein levels between the cytosolic and membrane fractions. Data represent the mean ± SE; *p<0.01 as compared to the obese control group. EX: Exercise Group; EX-in: Exercise + Inhibitor Group; AU: Arbitrary Unit.