Figure 2: Effect of AGEs and/or alendronate on osteoclastogenesis. (A) UMR- 106 osteoblastic cells and Raw 264.7 macrophages were co-cultured for 4 days until formation of multinucleated cells. They were then incubated with different doses (50 μg/ml, 100 μg/ml or 200 μg/ml) of BSA or AGEs for an additional 4 days. TRAP activity was determined in cell lysates by a colorimetric method. Results are expressed as % of basal (BSA alone), and are shown as the mean ± S.E.M. (* p<0.05, ** p<0.01). (B) UMR-106 osteoblastic cells and Raw 264.7 macrophages were co-cultured for 4 days until formation of multinucleated cells. They were then incubated for an additional 4 days with 100 μg/ml of BSA or AGEs, and/or different doses of alendronate (10-8M - 10-4M). TRAP activity was determined in cell lysates by a colorimetric method. Results are expressed as % of basal (BSA alone), and are shown as the mean ± S.E.M. (* p<0.05, ** p<0.001 versus BSA alone) (# p<0.001 versus AGEs alone)