-1722C>T -1661A>G -651C>T -318C>T +49 A>G -819C>T +6230G>A (CT60) Temp 1 (°C) 50.5 53 57.5 58 55 62 59 Duration 1 (s) 45 30 30 30 30 40 30 Temp 2 (°C) 72 72 72 72 72 72 72 Duration 2 (s) 40 30 30 30 30 30 30 bp fragment 398 214 330 212 152 180 216 Restriction enzyme APek1 DraI Aci1 Tru1 Ecor911 CviKl-I NcoI Source NEB FL NEB FL FL NEB FL Incubation temp (°C) 37 65 37 65 37 37 65 Digested allele CC AA CC TT AA CC AA Yielded fragments (bp) 266, 132 162 300 93, 119 131 69, 111 162 Digested allele TC AG CT CT AG CT AG Yielded fragments (bp) 132, 266, 398 52, 162, 214 300, 330 93, 119, 212 131, 152 69, 111, 180 54, 162, 216 Digested allele TT GG TT CC GG TT GG Yielded fragments (bp) 398 214 330 212 152 180 216 Abbreviations: NEB: New England Biolabs, UK; FL: Thermo Fisher Scientific, Inc (Fermentas), USA

Table 3: Details of SNP genotyping.