Treatments were started at 10 weeks of age and continued for 4 weeks. Compound 1was dosed at 100 mg/kg in vehicle, 0.25 % CMC containing Tween-80. N=8 for each group
A: Effect of compound 1 treatment on body weight in ob/ob mice. Body weights are shown in grams (g), and represent means ± SD; P values were calculated using repeated measures ANOVA procedure with post hoc testing. *P=0.04 for compound 1 versus vehicle (19 days); P=0.01 for compound-1 versus vehicle (26 days)
B: Effect of compound 1 on food consumption in ob/ob mice. Food intake was recorded daily during the study period. Shown is the average food intake in grams per mouse per day. Food intake over the study period (28 days) was averaged for each group
C: Evaluation of glucose tolerance in response to compound 1 treatment
Plasma glucose levels in an OGTT: OGTT was done at the end of 4 weeks of treatment. N=8 each for vehicle and compound 1 groups. Data shown are means ± SD. P values were calculated using the two sample, paired t test.*P=0.009 and P=0.02 and for compound-1 versus vehicle for 30 minute and 60 minute time points, respectively
D: Area under the curve (AUC) for OGTT shown in C. Total glucose area or area under the curve (AUC) was calculated by using the Excel spreadsheet and the formula, = SUMPRODUCT (A2: A20-A1:A19, (B2: B20+B1:B19) /2) where X values are in A1:A20 and Y values are in B1: B20. N= 8 for each group; P values were calculated using repeated measures ANOVA procedure with post hoc testing.* P= 0.03 for compound 1 versus vehicle groups
