Figure 5: Endothelial cell (HUVEC) cytoskeletal organization as a function of culture duration, shear exposure magnitude and albumin additive, as measured by immunofluorescence microscopy. All data are reported as the mean ± SEM of independent experiments for(A,B) actin structure, (C,D)actin alignment and (E,F) connectivity. * Significantly different from no added albumin and paired BSA (paired by shear stress and culture duration, three-way ANOVA, P< 0.05), + Significantly different than 0.4Pa (paired by additive and culture duration, three-way ANOVA, P< 0.05), # Significantly different than early (paired by shear stress and additive, three-way ANOVA, P< 0.05).