median normalisation

creatinine normalisation

 

2011

2010

2011

2010

metabolite

nr.

Bonferroni p

log2FC

error

Bonferroni p

log2FC

error

Bonferroni p

log2FC

error

Bonferroni p

log2FC

error

2-hydroxyisobutyrate

28

           

5.08398E-05

0.60

0.02

0.001640099

0.62

0.11

2-methylglutarate

20

           

8.90977E-06

0.48

0.01

     

2-oxoisocaproate

24

           

0.026346487

0.65

0.03

     

3-methylglutarate

       

0.000217485

-0.35

0.05

           

Alanine

1

2.30443E-07

1.12

0.03

0.000189398

0.95

0.14

3.79254E-07

1.44

0.03

7.95791E-05

1.10

0.15

Alloisoleucine

4

2.87668E-05

0.66

0.02

0.048853951

0.23

0.06

1.01314E-05

0.97

0.03

0.029174508

0.38

0.09

Betaine

16

0.004415538

-0.24

0.01

                 

Choline

10

0.041979148

0.37

0.02

     

0.000807888

0.68

0.03

     

Cis-aconitate

25

           

0.013382872

1.23

0.06

     

Creatinine

13

0.001866606

-0.31

0.01

                 

Dimethylamine

11

0.004785616

-0.29

0.01

                 

Ethanolamine*

12

0.000436376

-0.32

0.01

0.002733761

0.67

0.12

     

0.002348867

0.82

0.15

Glutamine

23

           

0.001535616

0.59

0.02

     

Glycylproline

26

           

0.023401534

0.59

0.03

     

Hippurate

8

8.7678E-06

-0.63

0.02

7.96473E-05

-0.69

0.09

0.030689263

-0.32

0.02

0.001228771

-0.57

0.10

Isoleucine

6

3.42392E-05

0.80

0.02

0.008592067

0.34

0.07

8.13268E-07

1.11

0.03

0.008068433

0.49

0.10

Lactate

9

0.010369908

0.54

0.03

     

3.76008E-05

0.85

0.03

0.012303742

0.72

0.15

Leucine

3

2.21915E-07

1.15

0.03

     

1.68387E-07

1.46

0.03

     

Malonate

                   

0.02242326

0.46

0.11

Methionine

22

           

0.029274138

0.62

0.03

     

Methylamine

18

0.002108651

-0.31

0.01

                 

Methylguanidine

19

6.26868E-05

-0.35

0.01

                 

N.N-dimethylglycine

21

           

0.044691484

0.27

0.02

     

N-acetylglutamate

17

0.000797992

-0.29

0.01

                 

N-isovaleroglycine

7

0.040530385

0.41

0.02

0.031514826

0.26

0.06

0.000924383

0.72

0.03

0.034029713

0.41

0.10

O-acetylcarnitine

       

0.020783386

0.29

0.06

     

0.012390392

0.42

0.09

Suberate

14

0.04311869

-0.68

0.04

0.00464694

-0.36

0.07

           

Succinate

27

     

0.012021138

0.49

0.11

0.007825682

0.57

0.03

0.003094892

0.64

0.12

Trimethylamine-N-oxide

15

0.00853877

-0.22

0.01

                 

Tyrosine

5

           

0.001960367

0.59

0.02

     

Valine

2

3.81256E-08

1.19

0.02

1.00024E-06

0.79

0.08

2.91723E-08

1.50

0.03

5.19345E-06

0.94

0.11

Table 1: Metabolites detected by NMR that changed following glucose infusion, normalised to either the sample median or corresponding creatinine concentration. Only metabolites with a p-value below 0.05 after Bonferroni correction (Bonferroni p) were accepted and no restriction was applied with regard to fold change (log2FC = log2 fold change, with baseline measurements as reference). Empty spaces represent detected, but non-(significantly) changing metabolites. *=inconsistent result for this metabolite.