Figure 6: Oxidative stress and damage. (A) Oxidative DNA damage. The 8-OHdG levels in Nrf2 WT and KO heart tissues were examined by immunofluorescent staining with an anti-8-OHdG antibody. (B) Lipid peroxidation. Nrf2 WT and KO heart tissues were homogenized and MDA production was measured. (C) Protein tyrosine nitrosation. Nrf2 WT and KO heart homogenates were immunoblotted with an anti-3NT antibody. Antisarcomeric acting was used as loading control. (D) Quantification of experiment (C).*, p<0.05; **, p<0.01.