Sample Amount of Protein(mg) Activity (nkat) Specific activity (U/mg) Purification Factor (fold) Yield (%) Crude enzyme 833.8 7505 9 1 100 Ammonium Sulfate precipitation 147.8 6802 46 5.1 90.60 Anion exchange chromatography 2.4 900 375 41.6 11.9

Table.2: Purification of azoreductase from Acinetobacter radioresistens.