Curing agents treatments concentration No. of colonies tested  from isolates Colonies lost antibiotic resistance Lab2 Lab11 Lab13 Lab2 Lab11 Lab13 (1) EtBr 10 µg/ml 50 50 50 0 0 0 (2) SDS 0.8 mg/ml 50 50 50 0 0 0 (3) EtBr at. 42°C 10 µg/ml 50 50 50 0 0 0 (4) SDS at 42°C 0.8 mg /ml 50 50 50 3 2 2

Table 2: Effect of different curing agents on cured plasmid conferring resistance to antibiotics in lactobacillus isolates.