| Enzymes |
Specific activity (nmol.min-1.mg-1 protein) in the cell-free extract,
prepared from cells grown on |
| PA |
4-HPA |
Glucose |
| PA-CoA ligase |
41 ± 7 |
nd |
nd |
| 4-HPAH |
30 ± 1 |
627 ± 19 |
nd |
| PAH |
nd |
nd |
nd |
| 3,4-DHPADO |
nd |
745 ± 10 |
nd |
| 2,5-DHPADO |
nd |
1.7 ± 0.3 |
nd |
Abbreviations: PA, Phenylacetic acid; 4-HPA, 4-hydroxyphenylacetic acid; PA-CoA
ligase, phenylacetyl-CoA ligase; 4-HPAH, 4-hydroxyphenylacetic acid hydroxylase;
PAH, phenylacetic acid hydroxylase; 3,4-DHPADO, 3,4-dihydroxyphenylacetic acid
dioxygenase; 2,5-DHPADO, 2,5-dihydroxyphenylacetic acid dioxygenase. Enzyme
activities were monitored as described in ‘Materials and Methods’. nd, no activity
detected. Values after ± indicate SD observed. |
