Figure 5: Phylogenetic classification of bacteria to the genus level in lysimeter 2 by tag encoded pyrosequencing of 16S rRNA amplicons (A) and metagenomic shotgun sequencing (B). Phylogenetic classification was performed using MG-RAST. For the 16S rRNA amplicon sequences, the parameters used were based on the Ribosomal Database (RDP release 10) with a maximum e-value of e-10, a minimum identity of 50% similarity, and a minimum alignment length of 50. For the metagenome shotgun sequences, data were compared to the M5NR database in MG-RAST using a maximum e-value of 1e-10, a minimum identity of 80%, and a minimum alignment length of 50. The green bars represent the sample DNA extracted in 2010 and the red bars represent the sample DNA extracted in 2011 after 4°C storage.