Assay Gene Target
(annealing temperature) |
Oligonucleotide Sequencesa |
nahA
(60°C) |
HK44NahAc182f: 5'-tttgtttgcagctatcacgg-3'
HK44NahAc302r: 5'-gcaaccgtagatgaagccat-3'
HK44NahAc263Taqr: 5'-(Fam)cgagcgacttctttcaaccccagac(BHQ1)-3' |
tetA
(60°C) |
HK44TetA1055f: 5'-AGGTGGATGAGGAACGTCAG-3'
HK44TetA1152r: 5'-ATAGATCGCCGTGAAGAGGA-3'
HK44TetA1102Taqr: 5'-(Fam)GACGATCGAGGTCAGGCTGGTGAG(BHQ1)-3' |
luxA
(60°C) |
HK44LuxA29f: 5'-ATCAACCACCAGGTGAAACTCA-3'
HK44LuxA167r: 5'-TCCCGTAAGACCAAACTCTGT-3'
HK44LuxATaq54f: 5'-(Fam)AAGTAATGGATCGCTTTGTTCGGCTTGGT(BHQ1)-3' |
a Present study. Probes were synthesized with FAM (fluorescein) and black hole quencher 1 (BHQ1) from Biosearch Technologies (Novato, CA, USA). |
