Parameters

Part of Plant used

Method of Analysis

Reference

Chlorophyll

Fresh leaves

Spectrophotometry

Yasidia et al.,1971

Carotenoid and Lycopene

Fresh leaves

Spectrophotometry

Zarkaria et al.,1979,Adsule &Ambadan,1979

Protein

Fresh leaves and Vegetables

Spectrophotometry

Lowry et al.,1951

Starch

Fresh leaves and Vegetables

Colorimetry

McCready et al.,1950

Ascorbic acid

Vegetables

Spectrophotometry

Roe & Keuther,1953

Reducing sugars

Vegetables

Colorimetry

Somogyi,1952

Total carbohydrates

Vegetables

Colorimetry

Hedge & Hefreiter,1962

Total soluble sugars

Vegetables

Colorimetry

Dubois,1956

Total phenol

Vegetables

Colorimetry

Bray&Thorpe,1954

Calcium

Vegetables

Titrimetry

Raghuramulu et al.,2003

Magnesium

Vegetables

Colorimetry

Raghuramalu et al.,2003

Table 1: Methodology for analysis of Biochemical Parameters in Leaves and Fruits.